Sumin Lee (Tomocube / Chief Scientist)
생명과학자 이박사는 어쩌다가 3차원 홀로그래피 회사의 프로젝트 매니저가 되었을까
QUANTITATIVE ANALYSIS OF CELL VOLUME AND THE DRY MASS IN A SINGLE CELL AND THE ORGANELLE DYNAMICS USING THREE-DIMENSIONAL QUANTITATIVE PHASE IMAGING
Quantitative Phase Imaging (QPI) provides label-free and real-time three-dimensional (3D) imaging capability. By using laser interferometry to measure 3-D refractive index (RI) distribution, 3D images of live cells without any molecular labeling can be obtained with high spatial resolution. Furthermore, QPI images can be analyzed to provide quantitative information of a single cell, including cell volume, dry mass, and protein concentration. In this seminar, we will discuss about the studies in quantitative cell biology by imaging the live cells with unprecedented correlative and quantitative bioimaging capabilities. We will also introduce representative studies which integrate artificial intelligent (AI) technologies for elucidating the characteristics based on the HT images and their quantitative information of individual mammalian cells.
 Park et al., Quantitative phase imaging in biomedicine, Nature Photonics 12, 578–589 (2018)
 Lee et al., Deep-learning based three-dimensional label-free tracking and analysis of immunological synapses of CAR-T cells, eLife 9:e49023 (2020)
 Choi et al., Label-free three-dimensional analyses of live cells with deep-learning-based segmentation exploiting refractive index distributions, BioRxiv 2021.05.23.445351 (2021)
Dr. Sumin Lee is the chief scientist and the team leader in Tomocube. Her team is running numerous research projects to explore biological applications using 3D quantitative phase imaging. Her main research interest is the quantitative analysis of individual cells and its organelle dynamics. She also has been managing the projects in product development including research instrument, in vitro diagnostic medical device, and 3D image analysis software. Dr. Lee received her Ph.D. degree from POSTECH in 2013 majoring Cellular Systems Biology. Until her post-doctoral training, she performed comparative studies of protein trafficking machineries to elucidate the evolution of organellar protein delivery mechanism in eukaryotic cells. From 2014 to 2017, She was a senior scientist in Division of Research Strategy Planning and Division of Forensic Genetics, National Forensic Service, Ministry of the Interior and Safety, Republic of Korea.